A protocol was devised for callus induction and in vitro micro propagation of Anisochilus carnosus. Leaf, nodal and immature inflorescence explants were cultured for callusing on solid MS medium with best callusing response achieved at higher concentrations of BAP (12mg/l) alone and in combination with 2,4-D (3mg/l) and IAA (5mg/l). Bud regeneration from inflorescence callus giving maximum (9.67±0.58) shoots per bud was achieved at 12mg/l BAP+5mg/l IAA. Elongated shoots were transferred to half strength MS liquid medium for in vitro rhizogenesis, with best rooting achieved in 2mg/l IBA giving maximum number of 13.00±1.00 roots per shoot. The rooted plantlets were hardened by transferring to polycups containing sterile soil and vermicompost in the ratio of 1:1. After 15 days of hardening, established plantlets were transferred to field with maximum survivability.
展开▼
机译：设计了一种方案用于肉食无足Anisochilus carnosus的愈伤组织诱导和体外微繁殖。在固体MS培养基上培养叶片，结节和未成熟的花序外植体，以达到最佳的愈伤反应，单独使用较高浓度的BAP（12mg / l）并与2,4-D（3mg / l）和IAA（5mg / l）。在12mg / l BAP + 5mg / l IAA的条件下，从花序愈伤组织中再生出芽，每个芽的芽数最多（9.67±0.58）。将伸长的芽转移到半强度MS液体培养基中以进行体外生根，在2mg / l IBA中获得最佳生根，每个芽最大根数为13.00±1.00。通过将生根的小植株以1：1的比例转移至含有无菌土壤和ver堆肥的多杯植物中进行硬化。硬化15天后，将已建立的幼苗以最大的生存能力转移到田间。
展开▼
