Histological studies of in vitro regeneration induced in leaf explants ofudNymphoides cristatum (Roxb) O.Kuntze – An aquatic medicinal plant

摘要

The objective of this work was to study the histological events related to the regeneration process of audmedicinal plant, Nymphoides cristatum (Roxb). Leaf explants were cultured on MS mediumudsupplemented with 0.5 mgl‐1 of 6‐benzyl amino purine (BAP). About 90% of explants gave rise to shootsudafter 15 days of incubation. The histological studies showed that the regeneration originated directlyudfrom parenchymatous cells and direct organogenesis after 20 days of culture could be observed. Budsudand roots were found completely differentiated after 40 days of culture and number of shoots perudexplants was 30. Micorshoots were rooted in hormone – free medium and the plants obtained grew inudartificial pond under green house conditions.