Pemanfaatan Sumber Karbohidrat Yang Berbeda (Umbi Suweg, Umbi Talas, Dan Umbi Kimpul) Sebagai Substitusi Media Na (Nutrient Agar) Untuk Pertumbuhan Bakteri

摘要

Instant media are difficult to obtain and relatively high prices encourage researchers to make a substitution media with materials that are cheap and easily. This studyaims to determinethe useof differentcarbohydrate sourcesassubstitutesmediaNutrientAgar (NA) forbacterial growth. Thisresearchusingcompletely randomized design(CRD) twofactors.The first factoris a typeof bacteriaisEscherichiacoliandStaphylococcusaureusandsecond is theAmorphophallus campanulatus, Colocasia esculenta, andXanthosoma violaceum. Inoculatedwith thetest bacteriaspreadplatemethodandincubatedat 37 °C for 24 hoursfor thebacteria inmediaNutrientagar and48 hourson mediumtubers.Parametersmeasured werethe number ofpopulationandthe size ofbacterial colonies.In Escherichiacoli, the highestpopulationon themediaof theAmorphophallus campanulatus (4.6 x 109CFU/ml), nutrient agar medium(3.6 x 109CFU/ml), Xanthosoma violaceum (3.5 x109CFU/ml), than the last Colocasia esculenta (2.8 x 109CFU/ml), while thehighestpopulation ofStaphylococcus aureuson theAmorphophallus campanulatus (8.2 x 109CFU/ml), nutrient agarmedia(5.5 x 109CFU/ml), Colocasia esculenta (3, 4x 109CFU/ml), and the last Xanthosoma violaceum (2.3 x 109CFU/ml). Based onthe size of thebacterial colonies, ofthe threemediatubersobtainsmallcolonybuta lot. The conclusion ofthis studymediathat can be usedas asubstitutenutrient agarmedium, ismediaofAmorphophallus campanulatus.