The effect of thawing fresh frozen plasma at various temperatures on in vitro coagulation factor activity

摘要

Thawing of fresh frozen plasma (FFP) in South Africa is not standardized and thawing at highudtemperatures may cause clotting factor activation and disseminated intravascular coagulationud(DIC). This research project studies the in-vitro effects of thawing FFP at variousudtemperatures on coagulation. Twenty units of FFP were each divided into 4 satellite bagsudwhich were respectively thawed at 22ºC, 37ºC, 45ºC and 60ºC and tested for Fibrinogen, DDimers,udPT, PTT, r value, Alpha Angle and Maximum Amplitude (MA). FFP thawed at 60ºCudshowed significant differences suggesting clotting factor inactivation. FFP thawed at 45ºCudshowed significantly elevated D-Dimers. Clotting factors thawed at 22ºC may be partiallyudinactivated. High thawing temperatures may activate and then denature the factors therein.udTwenty two degrees may partially inactivate FFP until it is warmed to body temperature. Theudclinical implications and recommendations of this study are to thaw FFP at 37ºC.