Background: Hand hygiene has a fundamental role in the prevention of one of the most challenging problems in the management of modern hospitals: nosocomial infections and occupational diseases.Aim: This survey presents the experience of laboratorians in the Microbiology Department in the Clinical Center in Skopje in promoting proper hand hygiene by participating in a training course and implementation of WHO guidelines among laboratory staff.Material and Methods: The group of 36 Health Care Workers (HCW) has been divided in to seven different groups, based on their specific work in the Institute of Microbiology. The investigation was conducted in two phases: phase-1 was before the training course and phase-2 immediately after taking the training course. During phase-2, hand samples were taken with the technique of the moist swab on the left hand and the fingertips touch method on the right hand.Results: It was found that 17% of the cultures from the left hand detected pathogenic bacteria and 39% on the samples taken from the right one (there is significant difference (p = 0.041).Conclusion: The Touch Method appears to be the more sensitive method than swabbing the critical areas of the palms. It is also evident (significant) that awareness of the problem of the staff, obtain some changes in their attitude and practice.
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