HPLC/DAD Intercomparison on Phytoplankton Pigments (HIP-1, HIP-2, HIP-3 and HIP-4)

摘要

From 2009 to 2015, in the context of the MERIS (Medium Resolution Imaging Spectrometer) validation activities, the JRC Marine Optical Laboratory organised four HPLC Intercomparison exercises for Phytoplankton Pigment measurements (HIP-1, HIP-2, HIP-3 and HIP-4), involving seven European accredited and reference laboratories.The objectives of these intercomparison exercises were: creating a reference community at European level for phytoplankton pigment analysis capable of supporting satellite data validation; quantifying single laboratory uncertainties; improving and maintaining the quality of results for a single laboratory with time and quantifying the differences among European laboratories applying published methods.The four intercomparisons have confirmed that chlorophyll a uncertainties requirement for satellite data validation activities (25% in oligotrophic water) are achievable for laboratories applying in HPLC phytoplankton pigment analysis.