Efeitos da intoxicação aguda com etanol sobre a exsudação de neutrófilos para a cavidade peritoneal de camundongos inoculados com Staphylococcus aureus

摘要

Introduction. There are indications that an acute intoxication with ethanol has anti-inflammatory effects, reducing the exudation of neutrophils and increasing susceptibility to infections, especially with Streptococcus pneumoniae. However we do not know the time these inhibitory effects of cellular exudation and reduced microbicidal activity of leukocytes persist after one acute intoxication with ethanol. .Objective. To evaluate the time of duration of the inhibitory effects of exudation of neutrophils and reduction of microbicidal activity in a model of peritonitis induced by Staphylococcus aureus after an acute ethanol intoxication in mice. Methods. C57BL/6 mice received by gavage one dose of 7mg of ethane/g body weight. Control animals received the same volume of distillated water. One, 12, 24, 48 and 72 hours after ethanol intoxication all mice received intra-peritoneal injection of Staphylococcus aureus (0.5 ml containing 6 to 9x108 CFU/mL of ATCC 25923 strain). Six hours later the animals were euthanized, and the peritoneal cavity rinsed with PBS / EDTA 0.01 M; one aliquot was used CFU counts and the other for global and specific count of cells in the exudates, using a Neubauer chamber and a cytospin to obtain smears that were stained by fast staining method with Dipquick. CFU counts were made by the method of serial dilution. Results. All animals receiving ethanol showed signs of intoxication, which reached a state of profound lethargy and all animals were recovering 45 minutes later.The ethanol dose used induced thymic involution, evident 24 hours after alcohol ingestion, with recovery being evident after 120 hours. In animals that received ethanol a significant reduction in the number of cells in exudates was observed; this reduction was due to reduction of exudation of neutrophils up to 24 hours after ethanol intoxication. The number of mononuclear cells were similar in both groups. Microscopic analysis of the smears showed bacteria out of the cells, more frequently in the ethanol group and the number of CFU was lower in this group but the observed difference was not statistically significant. Conclusion. Results confirm the anti-inflammatory effects of an acute ethanol intoxication, with a significant reduction in exudates of neutrophils, but with no significant changes in exudates of mononuclear leukocytes. The microbicidal activity of peritoneal cavity against staphylococci was lower in the ethanol group although without statistical significance, possibly because the resident macrophages were less affected by the effects of ethanol