Prozessentwicklung zur Produktion von 2-Keto-L-Gulonsäure, einer Vitamin C-Vorstufe

摘要

In this study a process for the continuous production of 2-keto-L-gulonic acid wasdeveloped. This compound is a precursor of vitamin C and therefore of great industrialinterest. 2-Keto-L-gulonic acid (2-KLG) was produced from D-glucose in a two-stepreaction: Glucose was first oxidized to 2,5-diketo-D-gluconic acid (2,5-DKG) with cells of Gluconobacter oxydans, 2,5-DKG was then reduced to 2-KLG with the enzyme2,5-diketo-D-gluconate reductase. Both reactions were carried out in separate reactors; 2,5-DKG was transferred into the second reactor without any further processing or purification....