Identification of mammalian lipid material by high performance liquid chromatography and Fourier transform ion cyclotron resonance mass spectrometry

摘要

The analysis of the exact compositions of human lipids is of great importance and canlead to a better understanding of the role of lipids in heart diseases and diabetes.Reverse phase and normal phase high performance liquid chromatography has beenused for the analysis of lipid material and applied to beef dripping. Electrosprayionization Fourier transform ion cyclotron resonance mass spectrometry has been usedto investigate the free fatty acids, monoacylglycerides, diacylglycerides andtriacylglycerides present in lard and beef dripping with the general conclusion that thisis a semi quantitative method. Collision induced dissociation of di- and triacylglycerideswas performed using Fourier transform ion cyclotron resonance mass spectrometry withthe energy of activation for the fragmentation process investigated and compared usingdensity functional theory and Rice Ramsperger Kassel Marcus statistical theory offragmentation. The application of tandem mass spectrometry to mice and human cellcultures and plaques from the abdominal aorta of rabbits was conducted using normalphase high performance liquid chromatography with electrospray ionization Fouriertransform ion cyclotron resonance mass spectrometry and quadrupole time of flightmass spectrometry.