首页> 外文OA文献 >Simple, low-cost molecular assays for TR34/L98H mutations in the cyp51A gene for rapid detection of triazole-resistant Aspergillus fumigatus isolates
【2h】

Simple, low-cost molecular assays for TR34/L98H mutations in the cyp51A gene for rapid detection of triazole-resistant Aspergillus fumigatus isolates

机译:cyp51A基因中TR34 / L98H突变的简单,低成本分子测定,可快速检测三唑抗性烟曲霉菌株

摘要

Simple, low-cost PCR/PCR-restriction fragment length polymorphism (RFLP) assays targeting cyp51A promoter and codon 98 regions were developed for the detection of triazole-resistant Aspergillus fumigatus strains carrying TR34/L98H mutations. The assays were evaluated using 40 itraconazole-susceptible isolates and 35 itraconazole-resistant isolates. The prevalence of TR34/L98H mutations in clinical/environmental A. fumigatus isolates may now be determined easily from resource-poor settings.
机译:开发了针对cyp51A启动子和98号密码子区域的简单,低成本PCR / PCR限制性片段长度多态性(RFLP)检测方法,用于检测携带TR34 / L98H突变的耐三唑烟曲霉菌株。使用40种伊曲康唑敏感性分离株和35种伊曲康唑抗性分离株对测定进行了评估。现在可以从资源匮乏的环境中轻松确定临床/环境烟曲霉菌株中TR34 / L98H突变的患病率。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号