The osteogenic differentiation of adipose tissue-derived precursor cells in A 3D scaffold/matrix environment

摘要

Abstract: This paper details an in-vitro study using human adipose tissue-derived precursor/stem cells (ADSCs) in three-dimensionalud(3D) tissue culture systems. ADSCs from 3 donors were seeded onto NaOH-treated medical grade polycaprolactone-tricalcium phosphateud(mPCL-TCP) scaffolds with two different matrix components; fibrin glue and lyophilized collagen. ADSCs within these scaffolds wereudthen induced to differentiate along the osteogenic lineage for a 28-day period and various assays and imaging techniques were performedudat Day 1, 7, 14, 21 and 28 to assess and compare the ADSC’s adhesion, viability, proliferation, metabolism and differentiation along theudosteogenic lineage when cultured in the different scaffold/matrix systems.udThe ADSC cells were proliferative in both collagen and fibrin mPCL-TCP scaffold systems with a consistently higher cell number (byudcomparing DNA amounts) in the induced group over the non-induced groups for both scaffold systems. In response to osteogenic induction,udthese ADSCs expressed elevated osteocalcin, alkaline phosphatase and osteonectin levels. Cells were able to proliferate within theudpores of the scaffolds and form dense cellular networks after 28 days of culture and induction.udThe successful cultivation of osteogenic by FDM process manufactured ADSCs within a 3D matrix comprising fibrin glue or collagen,udimmobilized within a robust synthetic scaffold is a promising technique which should enhance their potential usage in the regenerativeudmedicine arena, such as bone tissue engineering.