Application of hyperbaric oxygen in bone tissue engineering : effect of hyperbaric oxygen treatment on bone marrow stem cellsudud

摘要

and non-union of bony fractures has been proposed since 1966, little has been knownudabout the effect of HBOT on bone marrow stem cells (BMSC). The aim of this studyudis to investigate the effect of HBO treatment on osteogenetic differentiation of BMSCudand potential application in bone tissue engineering.udAdhesive stromal cells harvested from bone marrow were characterized byudmesenchymal differentiation potential, cell surface markers and their proliferationudcapacity. Mesenchymal stem cells, which demonstrated osteogenic, chondrogenic andudadipogenic differentiation potential and expressed positively for CD 29, CD 44, CDud73, CD 90, CD 105, CD 166 and negatively for CD34 and CD 45, were selected andudtreated in a laboratory-scale HBO chamber using different oxygen pressures andudexposure times. No obvious effect of HBO treatment on BMSC proliferation wasudnoticed. However, cytotoxic effects of HBO were considerably less pronounced whenudcells were cultured in medium supplemented with 10% FBS in comparison to mediumudsupplemented with 2% FCS, as was evaluated by WST-1 assay. Under HBOudtreatment, bone nodules were formed in three days, which was clearly revealed byudVon Kossa staining. In contrasts, without HBO treatment, bone nodules were notuddetected until 9-12 days using the same inducing culture media. Calcium depositionudwas also significantly increased after three days of HBO treatments compared to noudHBO treatment. In addition it was also found that oxygen played a direct role in theudenhancement of BMSC osteogenic differentiation, which was independent of theudeffect of air pressure.