Enteric pathogens from biofertilizer can accumulate in the soil, subsequentlyudcontaminating water and crops. We evaluated the survival, percolation and leachingudof model enteric pathogens in clay and sandy soils after biofertilization withudswine digestate: PhiX-174, mengovirus (vMC0), Salmonella enterica Typhimurium andudEscherichia coli O157:H7 were used as biomarkers. The survival of vMC0 and PhiX-174udin clay soil was significantly lower than in sandy soil (ıPT90 values of 10.520 0.600udvs. 21.270 1.100 and 12.040 0.010 vs. 43.470 1.300, respectively) and PhiX-ud174 showed faster percolation and leaching in sandy soil than clay soil (ıPT90 values ofud0.46 and 2.43, respectively). S. enterica Typhimurium was percolated and inactivatedudmore slowly than E. coli O157:H7 (ıPT90 values of 9.340 0.200 vs. 6.620 0.500udand 11.900 0.900 vs. 10.750 0.900 in clay and sandy soils, respectively), suchudthat E. coli O157:H7 was transferred more quickly to the deeper layers of both soilsudevaluated (percolation). Our findings suggest that E. coli O157:H7 may serve as a usefuludmicrobial biomarker of depth contamination and leaching in clay and sandy soil and thatudbacteriophage could be used as an indicator of enteric pathogen persistence. Our studyudcontributes to development of predictive models for enteric pathogen behavior in soils,udand for potential water and food contamination associated with biofertilization, useful forudrisk management and mitigation in swine digestate recycling.
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