Barley seed coating with free and immobilized alkaline phosphatase to improve P uptake and plant growth

摘要

Coating barley seeds with free and immobilized alkaline phosphatase was investigated as a potential means toudenhance plant utilization of accumulated soil phosphorus (P). Two coating techniques were studied: film-coatingudand pelleting. The highest phosphatase activity retention in the coating layer, ranging from 0·48 to 0·67, wasudobserved when seeds were film-coated with phosphatase–polyresorcinol complex (PPC). The germination ofudseeds film-coated or pelleted with alkaline phosphatase ranged from 0·84 to 0·97 or 0·14 to 0·25, respectively.udLow germination of the pelleted seeds was attributed to freezing the seeds in liquid nitrogen (N) for the layerudcoating formation. Pelleted seeds were not used in the remainder of the studies. Under pot culture conditions, anudincrease in the soil inorganic P was detected when the seeds were film-coated with phosphatase. Moreover, theudfilm-coating significantly increased the P uptake by plants (between 25 and 31% after 35 days after plantingud(DAP)). The present study showed that the seed film-coating with free and immobilized phosphatase increased theudphosphatase activity in the rhizosphere and the P uptake by plants.