Development of an ultra high performance liquid chromatographic-tandem mass spectrometric multi-analyte approach for target screening in eight different matrices and quantification in three different matrices

摘要

In the present dissertation, the development of a multi-analyte multi-biosample LC-MS/MS approach with simple extraction is described for the screening and identification of over 100 analytes in eight different matrices. The analytes were extracted with one single liquid-liquid work-up approach from eight different matrices and separated with one column and one multi-reaction mode (MRM) method. In addition, three methods for quantification of 19 benzodiazepines including Z-drugs, 33 antidepressants, and 33 neuroleptics in three different matrices were developed, fully validated, and the results evaluated concerning cross calibration. The present studies showed that matrix effects and recovery results in different biosamples have an influence on the cross calibration between these biosamples. The applicability of the presented method was shown using authentic human samples and external quality control samples. Especially for the group of antidepressants, it was shown that cross-calibration between different biosamples could only be used for some analytes.