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Does hydatid cyst fluid from Echinococcus granulosus cysts have any effect on cells involved in fibrosis in cystic echinococcosis?

机译:细粒棘球E囊肿的包虫囊肿液对囊性棘球cc病中与纤维化有关的细胞有任何影响吗?

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摘要

Cystic echinococcosis is characterized by the presence of slow growing hydatid cysts, usually in the liver or lungs. Survival of the parasite is based on an interaction of the host immune system and a range of parasite immune-evasive strategies. Fibrosis in the tissues surrounding the cysts can be seen as a host protective response isolating the parasite and restricting its growth or from another perspective fibrosis may be protective for the parasite by providing a barrier to more effective immunological responses. In this study the adenocarcinomic human alveolar basal epithelial cell line (A549) was used as model system. This cell line can be involved in fibrosis as cells can transform into mesenchymal cells and differentiate later to fibroblasts and/or myofibroblasts which can ultimately secrete collagen. Cells were initially cultured in vitro in RPMI-1640 medium containing 1-10% hydatid cyst fluid (HCF). The possible effect of the parasite extracts on cell migration was investigated using a wound healing assay. The ability of HCF components to modify cell surface markers of mesenchymal transition was also investigated by fluorescence microscopy. Results showed that there was a dose-dependent increase in cell growth in the presence of cyst fluid after 5 days of culture. The migratory response of cells was also enhanced by the presence of HCF. Both the enhanced growth and migratory activity were still evident when the HCF had been boiled indicating that the components responsible were thermostable. Semi-purified extracts of a major HCF component, antigen B showed a similar high stimulatory effect similar to that of HCF. The fluorescence microscopy showed a significant expression in the fibronectin and E-cadherin cell markers in cells treated with HCF. These results indicate that components within HCF have a stimulatory effect in the possible enhancement of fibrosis.
机译:囊性棘球cc病的特征是通常在肝脏或肺部存在缓慢生长的包虫囊肿。寄生虫的存活是基于宿主免疫系统和一系列寄生虫免疫逃避策略的相互作用。囊肿周围组织中的纤维化可以看作是隔离该寄生虫并限制其生长的宿主保护性反应,或者从另一个角度看,纤维化可以通过为更有效的免疫反应提供屏障来保护该寄生虫。在这项研究中,腺癌人类肺泡基底上皮细胞系(A549)被用作模型系统。该细胞系可参与纤维化,因为细胞可转变为间充质细胞,然后分化为最终可分泌胶原的成纤维细胞和/或成肌纤维细胞。首先在含有1-10%包虫囊液(HCF)的RPMI-1640培养基中体外培养细胞。使用伤口愈合试验研究了寄生虫提取物对细胞迁移的可能影响。还通过荧光显微镜研究了HCF组分修饰间充质转化的细胞表面标志物的能力。结果显示,培养5天后,在存在囊液的情况下,细胞生长呈剂量依赖性增加。 HCF的存在还增强了细胞的迁移反应。当HCF煮沸时,生长和迁徙活动的增强仍然很明显,这表明负责的成分是热稳定的。 HCF主要成分抗原B的半纯化提取物显示出与HCF相似的高刺激作用。荧光显微镜显示在用HCF处理的细胞中,纤连蛋白和E-钙粘着蛋白细胞标记物中有显着表达。这些结果表明,HCF中的成分在可能增强纤维化方面具有刺激作用。

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