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Comparative proteomic analysis of a potentially probiotic Lactobacillus pentosus MP-10 for the identification of key proteins involved in antibiotic resistance and biocide tolerance

机译:潜在益生菌戊糖乳杆菌MP-10的比较蛋白质组学分析,用于鉴定涉及抗生素抗性和杀生物剂耐受性的关键蛋白

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摘要

Probiotic bacterial cultures require resistance mechanisms to avoid stress-related responses under challenging environmental conditions; however, understanding these traits is required to discern their utility in fermentative food preparations, versus clinical and agricultural risk. Here, we compared the proteomic responses of Lactobacillus pentosus MP-10, a potentially probiotic lactic acid bacteria isolated from brines of naturally fermented Aloreña green table olives, exposed to sub-lethal concentrations of antibiotics (amoxicillin, chloramphenicol and tetracycline) and biocides (benzalkonium chloride and triclosan). Several genes became differentially expressed depending on antimicrobial exposure, such as the up-regulation of protein synthesis, and the down-regulation of carbohydrate metabolism and energy production. The antimicrobials appeared to have altered Lb. pentosus MP-10 physiology to achieve a gain of cellular energy for survival. For example, biocide-adapted Lb. pentosus MP-10 exhibited a down-regulated phosphocarrier protein HPr and an unexpressed oxidoreductase. However, protein synthesis was over-expressed in antibiotic- and biocide-adapted cells (ribosomal proteins and glutamyl-tRNA synthetase), possibly to compensate for damaged proteins targeted by antimicrobials. Furthermore, stress proteins, such as NADH peroxidase (Npx) and a small heat shock protein, were only overexpressed in antibiotic-adapted Lb. pentosus MP-10. Results showed that adaptation to sub-lethal concentrations of antimicrobials could be a good way to achieve desirable robustness of the probiotic Lb. pentosus MP-10 to various environmental and gastrointestinal conditions (e.g., acid and bile stresses).
机译:益生菌细菌培养需要抗性机制,以避免在充满挑战的环境条件下与压力相关的反应;但是,需要了解这些特征,以区分其在发酵食品制备中的用途,以及临床和农业风险。在这里,我们比较了戊糖乳杆菌MP-10的蛋白质组学响应,这是一种潜在的益生菌乳酸菌,是从自然发酵的阿罗尼亚绿色食用橄榄的盐水中分离出来的,暴露于低于致死浓度的抗生素(阿莫西林,氯霉素和四环素)和杀生物剂(苯扎氯铵)氯化物和三氯生)。取决于抗菌素暴露,一些基因变得差异表达,例如蛋白质合成的上调,碳水化合物代谢和能量产生的下调。抗菌药物似乎改变了Lb。戊糖MP-10的生理机能获得生存所需的细胞能量。例如,适应杀菌剂的Lb。戊糖MP-10具有下调的磷酸载体蛋白HPr和未表达的氧化还原酶。但是,蛋白质合成在适应抗生素和杀生物剂的细胞中过表达(核糖体蛋白和谷氨酰胺-tRNA合成酶),可能是为了补偿抗微生物剂靶向的受损蛋白。此外,应激蛋白,例如NADH过氧化物酶(Npx)和小的热激蛋白,仅在适应抗生素的Lb中过表达。戊糖MP-10。结果表明,适应亚致死浓度的抗菌剂可能是获得益生菌Lb理想耐用性的好方法。戊糖MP-10在各种环境和胃肠道条件下(例如酸和胆汁压力)。

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