On-Line Determination of Biomass in a Microalga Bioreactor Using a novel Computerized Flow Injection Analysis System

摘要

A flow injection analysis (FIA) device has been developed, which is able to assaysuccessfully for biomass in a microalga bioreactor. The device is fully computerizedand is operated via diluting small aliquots of the culture followed by measuring opticaldensity (OD); this figure is then accurately correlated with biomass, in terms of bothcell number and ash-free dry weight, during the entire culture time. Furthermore,the device is not expensive, is highly versatile, and is easy to operate owing tospecifically developed, user-friendly software. The growth rate and biomass productivityof Pavlova lutheri, cultivated under batch and semicontinuous modes, were monitoredas experimental testing model.