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The Responses of Ascorbate - Glutathione Cycle Enzymes in Seedlings of Pancratium maritimum L. under Drought Treatments

机译:干旱胁迫对Pan鱼幼苗抗坏血酸-谷胱甘肽循环酶的响应。

摘要

In this study, physiological and biochemical responses of (Pancratium maritimum L.), desert plant which is very widespread on coastal sand dunes to drought were determined. Therefore 28 days (d) old plants were drought stressed by withholding water for 5 and 10 days. The changes in relative growth rate (RGR), relative water content (RWC) lipid peroxidation, and ascorbate-glutathione cycle enzymes activity ((ascorbate peroxidase (APX, EC 1.11.1.11), glutathione reductase (GR, EC 1.6.4.2) dehydroascorbate reductase (DHAR, EC 1.8.5.1) and monodehydroascorbate reductase (MDAR, EC 1.6.5.4)) were investigated. Relative growth rate, relative water content were both decreased on the 5 and 10d of stress treatment while it was higher on the 10d. MDA content increased on the 10d while it did not change on the 5d. On the other hand, activities of APX, GR, DHAR and MDAR increased on the 5d but were not change on the 10d. These results suggest that ascorbate ? glutathione cycle enzymes were efficient to prevent from oxidative damage under short term of drought stress in (Pancratium maritimum L.) plants.
机译:在这项研究中,确定了沿海沙丘上非常普遍的沙漠植物(Pancratium maritimum L.)对干旱的生理和生化响应。因此,(d)28天的老植物因拒水5天和10天而遭受干旱胁迫。脱氢抗坏血酸相对生长速率(RGR),相对含水量(RWC)脂质过氧化和抗坏血酸-谷胱甘肽循环酶活性的变化((抗坏血酸过氧化物酶(APX,EC 1.11.1.11),谷胱甘肽还原酶(GR,EC 1.6.4.2)研究了还原酶(DHAR,EC 1.8.5.1)和单脱氢抗坏血酸还原酶(MDAR,EC 1.6.5.4),在胁迫处理的第5天和第10天,相对生长速率和相对含水量均降低,而在第10天时则相对较高。 MDA含量在10d时增加,而在5d时没有变化;另一方面,APX,GR,DHAR和MDAR的活性在5d时增加,但在10d时没有变化,这表明抗坏血酸,谷胱甘肽循环酶(Pancratium maritimum L.)植物在短期干旱胁迫下有效预防氧化损伤。

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