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Dehydrin-like proteins in the necrotrophic fungus Alternaria brassicicola have a role in plant pathogenesis and stress response

机译:坏死性真菌Alternaria braciicicola中的脱水素样蛋白在植物发病机理和胁迫响应中起作用

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摘要

In this study, the roles of fungal dehydrin-like proteins in pathogenicity and protection against environmental stresses were investigated in the necrotrophic seed-borne fungus Alternaria brassicicola. Three proteins (called AbDhn1, AbDhn2 and AbDhn3), harbouring the asparagine-proline-arginine (DPR) signature pattern and sharing the characteristic features of fungal dehydrin-like proteins, were identified in the A. brassicicola genome. The expression of these genes was induced in response to various stresses and found to be regulated by the AbHog1 mitogen-activated protein kinase (MAPK) pathway. A knock-out approach showed that dehydrin-like proteins have an impact mainly on oxidative stress tolerance and on conidial survival upon exposure to high and freezing temperatures. The subcellular localization revealed that AbDhn1 and AbDhn2 were associated with peroxisomes, which is consistent with a possible perturbation of protective mechanisms to counteract oxidative stress and maintain the redox balance in AbDhn mutants. Finally, we show that the double deletion mutant Delta Delta abdhn1-abdhn2 was highly compromised in its pathogenicity. By comparison to the wild-type, this mutant exhibited lower aggressiveness on B. oleracea leaves and a reduced capacity to be transmitted to Arabidopsis seeds via siliques. The double mutant was also affected with respect to conidiation, another crucial step in the epidemiology of the disease.
机译:在这项研究中,研究了在坏死性种子传播的真菌Alternaria braciicicola中真菌脱水蛋白样蛋白在致病性和对环境胁迫的保护中的作用。在芸苔假单胞菌基因组中鉴定了三种蛋白(称为AbDhn1,AbDhn2和AbDhn3),它们具有天冬酰胺-脯氨酸-精氨酸(DPR)签名模式,并具有真菌类脱水蛋白样蛋白的特征。这些基因的表达被诱导响应各种压力,并发现由AbHog1丝裂原活化蛋白激酶(MAPK)通路进行调节。敲除方法显示,脱水素样蛋白主要影响氧化应激耐受性以及暴露于高温和冰冻温度下对分生孢子的存活率。亚细胞定位揭示AbDhn1和AbDhn2与过氧化物酶体相关,这与可能的扰动保护机制以抵消氧化应激并维持AbDhn突变体中的氧化还原平衡有关。最后,我们显示了双缺失突变体Delta Delta abdhn1-abdhn2在其致病性上受到了很大的影响。与野生型相比,该突变体对油菜双歧杆菌叶片表现出较低的侵略性,并且通过长角果传递给拟南芥种子的能力降低。双重突变体的受胎情况也受到影响,这是该病流行病学中的另一个关键步骤。

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