Electrospun polylactic acid and polyvinyl alcohol fibers as efficient and stable nanomaterials for immobilization of lipases

摘要

Electrospinning was applied to create easy-to-handle and high-udsurface-area membranes from continuous nanofibers of polyvinyl udalcohol (PVA) or polylactic acid (PLA). Lipase PS from udBurkholderia cepacia and Lipase B from Candida antarctica (CaLB) udcould be immobilized effectively by adsorption onto the fibrous udmaterial as well as by entrapment within the electrospun udnanofibers. The biocatalytic performance of the resulting udmembrane biocatalysts was evaluated in the kinetic resolution of udracemic 1-phenylethanol (rac-1) and 1-phenylethyl acetate (rac-ud2). Fine dispersion of the enzymes in the polymer matrix and udlarge surface area of the nanofibers resulted in an enormous udincrease in the activity of the membrane biocatalyst compared to udthe non-immobilized crude powder forms of the lipases. PLA as udfiber-forming polymer for lipase immobilization performed better udthan PVA in all aspects. Recycling studies with the various udforms of electrospun membrane biocatalysts in ten cycles of the udacylation and hydrolysis reactions indicated excellent stability udof this forms of immobilized lipases. PLA-entrapped lipases udcould preserve lipase activity and enantiomer selectivity much udbetter than the PVA-entrapped forms. The electrospun membrane udforms of CaLB showed high mechanical stability in the repeated udacylations and hydrolyses than commercial forms of CaLB udimmobilized on polyacrylamide beads (Novozyme 435 and IMMCALB-udT2-150). © 2016 Springer-Verlag Berlin Heidelberg