Bio-orthogonal Fluorescent Labelling of Biopolymers through Inverse-Electron-Demand Diels–Alder Reactions

摘要

Bio-ortudhogonaudllabellinudgschemesudbasedudon inverse-elecudtron-uddemanuddDiels–Alduder (IEDDA)udcycloauddditionudhaveudattractedudmuchudattentionudin chemudical biologyudrecentlyud.The appealudingudfeaturesudof this reactioudn, suchudas the fast reactioudnkinetics,udfullyudbio-ortudhogonaludnatureudand highudselectivudity,have helpedudchemudi-udcal biologistsudgainuddeeperudunderstandiudng of biochemicudal pro-udcessesudat the molecularudlevel.Listingudthe compoudnentsudand dis-udcussingudthe possibudilitiesudandlimitationsudof thesereagentuds, weudprovidudearecentudsnapshotudof the fieldudof IEDDAud-basedudbiomo-udlecularudmanipulatioudnwith specialudfocusudon fluoresudcentudmodula-udtionudapproachesudthrougudhthe use of bio-orthogonudalizedudbuild-uding blocks.udAt the end,udwe discussudchallengesudthatudneedudto beudaddresudsedudfor furtheruddevelopudmentsudin orderudto overcomeudrecentudlimitaudtionsudand to enabluderesearchersudto answerudbiomoud-udlecularudquestudions in moreuddetail.