Anti-irritant and anti-inflammatory effects of glycerol andudxylitol in sodium lauryl sulphate-induced acute irritation

摘要

Background Glycerol is known to possess anti-irritant and hydrating properties and previous studies suggested thatudxylitol may also have similar effects.udObjective Our aim was to study whether different concentrations of these polyols restore skin barrier function andudsoothe inflammation in sodium lauryl sulphate (SLS)-induced acute irritation.udMethods The experiments were performed on male SKH-1 hairless mice. The skin of the dorsal region was exposed toudSLS (5%) for 3 h alone or together with 5% or 10% of glycerol respectively. Further two groups received xylitol solutionsud(8.26% and 16.52% respectively) using the same osmolarities, which were equivalent to those of the glycerol treatments.udThe control group was treated with purified water. Transepidermal water loss (TEWL) and skin hydration were determined.udMicrocirculatory parameters of inflammation were observed by means of intravital videomicroscopy (IVM). Furthermore,udaccumulation of neutrophil granulocytes and lymphocytes, the expression of inflammatory cytokines and SLSudpenetration were assessed, as well.udResults Treatment with the 10% of glycerol and both concentrations of xylitol inhibited the SLS-induced elevation ofudTEWL and moderated the irritant-induced increase in dermal blood flow and in the number of leucocyte-endothelial interactions.udAll concentrations of the applied polyols improved hydration and prevented the accumulation of lymphocytesudnear the treatment site. At the mRNA level, neither glycerol nor xylitol influenced the expression of interleukin-1 alpha.udHowever, expression of interleukin-1 beta was significantly decreased by the 10% glycerol treatment, while expressionudof tumour necrosis factor-alpha decreased upon the same treatment, as well as in response to xylitol. Higher polyoludtreatments decreased the SLS penetration to the deeper layers of the stratum corneum.udConclusion Both of the analysed polyols exert considerable anti-irritant and anti-inflammatory properties, but theudeffective concentration of xylitol is lower than that of glycerol.