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The Use of Carboxyfluorescein Diacetate Succinimidyl Ester (CFSE) to Monitor Lymphocyte Proliferation

机译:羧基荧光素二乙酸琥珀酰亚胺酯(CFSE)在监测淋巴细胞增殖中的应用

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摘要

Carboxyfluorescein succinimidyl ester (CFSE) is an effective and popular means to monitor lymphocyte division1-3. CFSE covalently labels long-lived intracellular molecules with the fluorescent dye, carboxyfluorescein. Thus, when a CFSE-labeled cell divides, its progeny are endowed with half the number of carboxyfluorescein-tagged molecules and thus each cell division can be assessed by measuring the corresponding decrease in cell fluorescence via Flow cytometry. The capacity of CFSE to label lymphocyte populations with a high fluorescent intensity of exceptionally low variance, coupled with its low cell toxicity, make it an ideal dye to measure cell division. Since it is a fluorescein-based dye it is also compatible with a broad range of other fluorochromes making it applicable to multi-color flow cytometry. This article describes the procedures typically used for labeling mouse lymphocytes for the purpose of monitoring up to 8 cell divisions. These labeled cells can be used both for in vitro and in vivo studies.
机译:羧基荧光素琥珀酰亚胺酯(CFSE)是监测淋巴细胞分裂1-3的有效且流行的手段。 CFSE用荧光染料羧基荧光素共价标记长寿命的细胞内分子。因此,当CFSE标记的细胞分裂时,其后代被赋予一半数量的羧基荧光素标记分子,因此,每个细胞分裂都可以通过流式细胞术通过测量细胞荧光的相应降低来评估。 CFSE能够以极低的变异性的高荧光强度标记淋巴细胞群体,并具有低细胞毒性,使其成为测量细胞分裂的理想染料。由于它是基于荧光素的染料,因此还与多种其他荧光染料兼容,使其适用于多色流式细胞仪。本文介绍了通常用于标记小鼠淋巴细胞的程序,目的是监视多达8个细胞分裂。这些标记的细胞可用于体外和体内研究。

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