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A direct binding assay for rheumatoid factor serum antiglobulins using fluorescein-labelled Fc fragment of human immunoglobulin-G.

机译:使用人免疫球蛋白-G的荧光素标记的Fc片段对类风湿因子血清抗球蛋白进行直接结合测定。

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摘要

A simple routine method is described for the quantitation of antiglobulin (rheumatoid factor) in human serum irrespective of its immunoglobulin class. Papain Fc fragment of human IgG is labelled with fluorescein isothiocyanate and incubated with the serum. Bound Fc is separated from free Fc by precipitation with polyethylene glycol and measured by the fluorescence of the precipitate. Results are expressed as a percentage of the binding obtained with a normal pool serum. About 60% of seronegative rheumatoid sera and 96% of seropositive rheumatoid sera gave results more than 2SD above the mean value for normal sera. Only 17% of osteoarthritis sera gave positive results by the same criterion. A relationship is also deduced to show that if the test is carried out at a number of Fc concentrations a double reciprocal plot of bound Fc against free Fc enables both the total antiglobulin and the ratio of binding constants of antiglobulin for Fc and for IgG to be calculated. This ratio is approximately unity. Under the standard conditions the results correspond to a measurement of approximately 30% of the total antiglobulins present.
机译:描述了一种简单的常规方法,用于定量人血清中的抗球蛋白(类风湿因子),无论其免疫球蛋白类别如何。用异硫氰酸荧光素标记人IgG的木瓜蛋白酶Fc片段,并与血清一起孵育。通过用聚乙二醇沉淀将结合的Fc与游离的Fc分离,并通过沉淀的荧光测量。结果表示为用正常合并血清获得的结合的百分比。血清阴性类风湿血清和血清阳性类风湿血清约60%的结果比正常血清平均值高2SD以上。根据相同的标准,只有17%的骨关节炎血清给出阳性结果。还推导了一种关系,表明如果在一定数量的Fc浓度下进行测试,则结合的Fc与游离Fc的双倒数图可使总抗球蛋白和抗球蛋白对Fc和IgG的结合常数之比为计算。该比率大约为1。在标准条件下,结果相当于存在的总抗球蛋白的约30%。

著录项

  • 作者

    Singh, I; Francis, G E;

  • 作者单位
  • 年度 1978
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  • 原文格式 PDF
  • 正文语种 en
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