首页> 外文OA文献 >Effects of cytochalasin B, colchicine and vincristine on the metabolism of isolated fat-cells
【2h】

Effects of cytochalasin B, colchicine and vincristine on the metabolism of isolated fat-cells

机译:细胞松弛素B,秋水仙碱和长春新碱对分离的脂肪细胞代谢的影响

代理获取
本网站仅为用户提供外文OA文献查询和代理获取服务,本网站没有原文。下单后我们将采用程序或人工为您竭诚获取高质量的原文,但由于OA文献来源多样且变更频繁,仍可能出现获取不到、文献不完整或与标题不符等情况,如果获取不到我们将提供退款服务。请知悉。

摘要

1. Colchicine and vincristine only slightly inhibit the metabolism of glucose to CO2 and lipids by isolated fat-cells. 2. Prolonged incubation with these agents causes no further inhibition. 3. Cytochalasin B, however, inhibits glucose metabolism to both CO2 and lipids in fat-cells. 4. However, at a concentration that causes a strong inhibition of glucose metabolism cytochalasin B is without effect on the metabolism of pyruvate, lactate or arginine to these end products. The uptake of labelled α-aminoisobutyrate is likewise not modified. Similarly it does not affect release of glycerol or free fatty acid, or the actions of adrenaline, insulin or caffeine on these parameters. At 10μg/ml it slightly lowers ATP concentrations, an effect that does not occur at 2μg/ml. 5. The transport of fructose into adipocytes by a specific fructose-transport system is also not affected by the agent, but the uptake of 2-deoxyglucose is strongly inhibited. It is concluded that cytochalasin B may specifically inhibit the glucose-transport system of isolated fat-cells. 6. Cytochalasin A has a much weaker action than cytochalasin B on glucose metabolism.
机译:1.秋水仙碱和长春新碱只能通过分离的脂肪细胞轻微抑制葡萄糖代谢为CO2和脂质。 2.与这些药物长时间孵育不会引起进一步的抑制作用。 3.然而,细胞松弛素B抑制葡萄糖代谢为脂肪细胞中的CO2和脂质。 4.但是,在引起强烈抑制葡萄糖代谢的浓度下,细胞松弛素B对丙酮酸,乳酸或精氨酸向这些终产物的代谢没有影响。标记的α-氨基异丁酸酯的摄取同样未改变。同样,它不影响甘油或游离脂肪酸的释放,也不影响肾上腺素,胰岛素或咖啡因对这些参数的作用。当浓度为10μg/ ml时,它会稍微降低ATP浓度,而浓度为2μg/ ml时不会发生这种作用。 5.通过特定的果糖转运系统将果糖转运到脂肪细胞中也不受该试剂的影响,但是强烈抑制了2-脱氧葡萄糖的摄取。结论是细胞松弛素B可能特异性抑制分离的脂肪细胞的葡萄糖转运系统。 6.细胞松弛素A在葡萄糖代谢上的作用比细胞松弛素B弱得多。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
代理获取

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号