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Enumeration of micro-organisms in food: a comparative study of five methods.

机译:食品中微生物计数:五种方法的比较研究。

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摘要

Five methods for the enumeration of micro-organisms in food (pour plate, surface spread plate, surface drop, agar droplet, and microdilution) were used in parallel to examine 100 samples selected from a wide range of food products. Statistical analyses of the results showed that the regression and correlation coefficients between the methods were highly significant; the lowest correlation coefficient derived for any pair of methods was 0.979. A variation in count between the methods of less than 0.5 log10 cycles was given by 98% of the samples. Analysis of the operation times and material requirements of each method showed that substantial savings in cost, time, space and support labour were achieved with the microdilution and agar droplet techniques as compared with the conventional pour plate and spread plate methods.
机译:并行使用五种食品中微生物计数方法(倾倒板,表面摊铺板,表面下降,琼脂滴和微稀释),以检查选自多种食品的100个样品。结果的统计分析表明,两种方法之间的回归系数和相关系数非常显着。任何一对方法得出的最低相关系数均为0.979。在98%的样品中,方法之间的计数差异小于0.5 log10个循环。对每种方法的操作时间和材料要求进行的分析表明,与传统的倾板法和铺板法相比,微稀释和琼脂滴技术可以节省大量的成本,时间,空间和辅助劳动。

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