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Rapid diagnosis of bacteremia in adults using acridine orange stained buffy coat smears

机译:使用a啶橙染色的血沉棕黄层涂片快速诊断成人菌血症

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摘要

The use of acridine orange stained buffy coat smears was assessed as a rapid screening test for bacteremia in adults. A total of 356 consecutive blood cultures were submitted with simultaneous anticoagulated blood samples, from which a buffy coat smear was prepared and stained with acridine orange (100 mg/L; pH 3.0). Forty-one of 356 blood samples (12%) yielded organisms in the blood culture system. Compared to blood culture, the overall sensitivity of acridine orange stained buffy coat smears was 16%, specificity 88%, and positive predictive value 13%. There was no statistically significant difference in performance of the test among patients who had fever greater than 39°C and/or shock. The low sensitivity and specificity of the test makes it unsuitable as a means of rapid screening for adults with suspected bacteremia.
机译:评估使用cr啶橙染色的血沉棕黄层涂片作为成人菌血症的快速筛查测试。总共进行了356次连续血液培养,同时进行了抗凝血样,从中制备了血沉棕黄层涂片并用a啶橙(100 mg / L; pH 3.0)染色。 356个血液样本中有41个(12%)在血液培养系统中产生了生物。与血液培养相比,of啶橙染色的血沉棕黄层涂片的总体敏感性为16%,特异性为88%,阳性预测值为13%。在发烧高于39°C和/或休克的患者中,测试表现没有统计学上的显着差异。该测试的低灵敏度和特异性使其不适合作为可疑菌血症成人的快速筛查手段。

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