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CELLULAR DIFFERENTIATION OF THE IMMUNE SYSTEM OF MICE : I. SEPARATE SPLENIC ANTIGEN-SENSITIVE UNITS FOR DIFFERENT TYPES OF ANTI-SHEEP ANTIBODY-FORMING CELLS

机译:小鼠免疫系统的细胞分化:I.不同类型的抗绵羊抗体形成细胞的分离脾抗原敏感单位

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摘要

Spleen cell suspensions of unprimed donor mice containing precursors of immunocytes have been transplanted into X-irradiated recipient mice. In the presence of antigen (sheep erythrocytes) these precursors, called antigen-sensitive units, gave rise to progeny cells secreting specific antibody. We studied quantitatively the production of cells releasing IgM hemolysins (direct plaque-forming cells), IgG hemolysins (indirect plaque-forming cells), and hemagglutinins (cluster-forming cells). We found that each of these immunocyte populations was distinct, i.e., that cells releasing agglutinins did not, as a rule, release hemolysins, and vice versa. We also found that cell populations secreting IgM hemolysins did not shift, under certain experimental conditions, to the production of IgG hemolysins during the primary immune response. By transplanting graded numbers of spleen cells, we succeeded in limiting to one or a few the number of antigen-sensitive units that reached the recipient spleen. We estimated thereby the frequency of antigen-sensitive units in donor cell suspensions and tested their potential for production of immunocytes of more than one type. Our results indicated that antigen-sensitive units were unipotent for they displayed in the spleens of unprimed donors the same restrictions of function and heterogeneity (antibody-specificity differentiation, antibody-class differentiation) found among antibody-forming cells. Furthermore, antigen-sensitive precursors for direct plaque-forming cells, indirect plaque-forming cells, and cluster-forming cells were detected in the spleens of unprimed mice in different frequencies, i.e., 1 in ∼ 106, 1 in ∼ 7 x 106, and 1 in ∼ 19 x 106 spleen cells, respectively. We concluded that relatively advanced differentiation of potentially competent cells occurs before sheep erythrocyte administration. The relevance of this finding for the broad spectrum of immunologic reactivities and for the heterogeneity of antibody responses to given antigens was discussed.
机译:含有免疫细胞前体的未启动供体小鼠的脾细胞悬液已被移植到X射线照射的受体小鼠中。在存在抗原(绵羊红细胞)的情况下,这些称为抗原敏感单元的前体产生了分泌特异性抗体的子代细胞。我们定量研究了释放IgM溶血素(直接噬菌斑形成细胞),IgG溶血素(间接噬菌斑形成细胞)和血凝素(形成团簇的细胞)的细胞产生。我们发现每个这些免疫细胞群是不同的,即释放凝集素的细胞通常不释放溶血素,反之亦然。我们还发现,在某些实验条件下,分泌IgM溶血素的细胞群体在一次免疫应答过程中并未转移至IgG溶血素的产生。通过移植分级数量的脾细胞,我们成功地将到达受体脾的抗原敏感单位的数目限制为一个或几个。因此,我们估计了供体细胞悬液中抗原敏感单位的频率,并测试了它们产生一种以上免疫细胞的潜力。我们的结果表明,抗原敏感单位是单能的,因为它们在未引发供体的脾脏中显示出在抗体形成细胞中发现的相同的功能和异质性限制(抗体特异性分化,抗体类别分化)。此外,在未引发小鼠的脾脏中,以不同的频率(即,约106的1处,约7 x 106的1处)检测到直接噬菌斑形成细胞,间接噬菌斑形成细胞和簇形成细胞的抗原敏感前体。和分别约19 x 106个脾细胞中的1个。我们得出结论,在绵羊红细胞给药之前,潜在感受态细胞发生了相对高级的分化。讨论了这一发现与广泛的免疫反应性和对给定抗原的抗体反应异质性的相关性。

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