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Molecular Diversity of Renibacterium salmoninarum Isolates Determined by Randomly Amplified Polymorphic DNA Analysis

机译:随机扩增多态性DNA分析确定沙门氏菌分离株的分子多样性。

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摘要

The molecular diversity among 60 isolates of Renibacterium salmoninarum which differ in place and date of isolation was investigated by using randomly amplified polymorphic DNA (RAPD) analysis. Isolates were grouped into 21 banding patterns which did not reflect the biological source. Four 16S-23S rRNA intergenic spacer (ITS1) sequence variations and two alleles of an exact tandem repeat locus, ETR-A, were the bases for formation of distinct groups within the RAPD clusters. This study provides evidence that the most common ITS1 sequence variant, SV1, possesses two copies of a 51-bp repeat unit at ETR-A and has been widely dispersed among countries which are associated with mainstream intensive salmonid culture.
机译:使用随机扩增多态性DNA(RAPD)分析技术,研究了60株鲑鱼雷尼菌分离株的分子多样性,这些分离株的分离地点和日期不同。分离物被分为21个带状图谱,这些带状图不反映生物学来源。四个16S-23S rRNA基因间隔子(ITS1)序列变异和确切的串联重复基因座ETR-A的两个等位基因是在RAPD簇内形成不同基团的基础。这项研究提供了证据,即最常见的ITS1序列变异体SV1在ETR-A处具有两个拷贝的51 bp重复单元,并且已广泛散布于与主流密集鲑鱼养殖有关的国家中。

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