首页> 外文OA文献 >Sequences of the joining region genes for immunoglobulin heavy chains and their role in generation of antibody diversity.
【2h】

Sequences of the joining region genes for immunoglobulin heavy chains and their role in generation of antibody diversity.

机译:免疫球蛋白重链连接区基因的序列及其在抗体多样性产生中的作用。

代理获取
本网站仅为用户提供外文OA文献查询和代理获取服务,本网站没有原文。下单后我们将采用程序或人工为您竭诚获取高质量的原文,但由于OA文献来源多样且变更频繁,仍可能出现获取不到、文献不完整或与标题不符等情况,如果获取不到我们将提供退款服务。请知悉。

摘要

To assess the contribution to immunoglobulin heavy chain diversity made by recombination between variable region (VH) genes and joining region (JH) genes, we have determined the sequence of about 2000 nucleotides spanning the rearranged JH gene cluster associated with the VH gene expressed in plasmacytoma HPC76. The active VH76 gene has recombined with the second germ-line JH gene. The region we have studied contains two other JH genes, designated JH3 and JH4. No other JH gene was found within the region 1000 nucleotides downstream from JH4. Between JH3 and JH4 there is a pseudo-JH sequence with substantial homology to the authentic JH genes. The four JH genes whose sequences now are known can account for all known JH amino acid sequences. The JH genes are more divergent than the Jk genes and vary in length, encoding either 15 or 17 amino acid residues. Because JH regions comprise part of the third hypervariable region (HV3), combinatorial VH--JH joining substantially augments VH diversity. Moreover, a VH gene can recombine with each JH gene at several positions, and either one or two germ-line JH codons can be excised. This JH truncation markedly reduces the length of HV3 and hence must alter antigen-binding specificity. We have also determined the sequence of the JH4 region in two different gamma 2a mRNAs and have found that each has suffered a point mutation (aspartate to asparagine) which would alter the charge of the antigen-binding site.
机译:为了评估可变区(VH)基因和连接区(JH)基因之间重组对免疫球蛋白重链多样性的贡献,我们确定了约2000个核苷酸的序列,该序列跨越与浆细胞瘤中表达的VH基因相关的重排JH基因簇HPC76。活性VH76基因已与第二个种系JH基因重组。我们研究的区域包含另外两个JH基因,分别称为JH3和JH4。在JH4下游1000个核苷酸的区域内未发现其他JH基因。在JH3和JH4之间,存在一个与真实JH基因具有实质同源性的伪JH序列。现在已知其序列的四个JH基因可以解释所有已知的JH氨基酸序列。 JH基因比Jk基因差异更大,并且长度不同,编码15个或17个氨基酸残基。因为JH区构成了第三高变区(HV3)的一部分,所以组合式VH-JH连接大大增强了VH分集。此外,VH基因可以与每个JH基因在几个位置重组,并且可以切除一个或两个种系JH密码子。 JH截短显着减少了HV3的长度,因此必须改变抗原结合的特异性。我们还确定了两个不同的γ2amRNA中JH4区的序列,并发现每个均遭受了点突变(天冬氨酸转化为天冬酰胺),这会改变抗原结合位点的电荷。

著录项

  • 作者

    Gough, N M; Bernard, O;

  • 作者单位
  • 年度 1981
  • 总页数
  • 原文格式 PDF
  • 正文语种 en
  • 中图分类

相似文献

  • 外文文献
  • 中文文献
  • 专利
代理获取

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号