首页> 外文OA文献 >The antibody response to myoglobin is independent of the immunized species. Analysis in terms of replacements in the antigenic sites and in environmental residues of the cross-reactions of fifteen myoglobins with sperm-whale myoglobin antisera raised in different species.
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The antibody response to myoglobin is independent of the immunized species. Analysis in terms of replacements in the antigenic sites and in environmental residues of the cross-reactions of fifteen myoglobins with sperm-whale myoglobin antisera raised in different species.

机译:对肌红蛋白的抗体反应独立于免疫物种。分析了十五种肌球蛋白与不同物种中精子-鲸鱼肌红蛋白抗血清的交叉反应的抗原部位和环境残基中的替代物。

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摘要

The recent determination of the entire antigenic structure of sperm-whale myoglobin with rabbit and goat antisera has permitted the examination of whether the antigenic structure recognized by antibodies depends on the species in which the antisera are raised. Also, by knowledge of the antigenic structure, the molecular factors that determine and influence antigenicity can be better understood in terms of the effects of amino acid substitutions occurring in the antigenic sites and in the environmental residues of the sites. In the present work, the myoglobins from finback whale, killer whale, horse, chimpanzee, sheep, goat, bovine, echidna, viscacha, rabbit, dog, cape fox, mouse and chicken were examined for their ability to cross-react with antisera to sperm-whale myoglobin. By immunoadsorbent titration studies with radioiodinated antibodies, each of these myoglobins was able to bind antibodies to sperm-whale myoglobin raised in goat, rabbit, chicken, cat, pig and outbred mouse. It was found that the extent of cross-reaction of a given myoglobin was not dependent on the species in which the antisera were raised. This indicated that the antibody response to sperm-whale myoglobin (i.e. its antigenic structure) is independent of the species in which the antisera are raised and is not directed to regions of sequence differences between the injected myoglobin and the myoglobin of the immunized host. Indeed, in each antiserum from a given species examined, that antiserum reacted with the myoglobin of that species. The extent of this auto-reactivity for a given myoglobin was comparable with the general extent of cross-reactivity shown by that myoglobin with antisera raised in other species. The cross-reactivities and auto-reactivities (both of which are of similar extents for a given myoglobin) can be reasonably rationalized in terms of the effects of amino acid substitutions within the antigenic sites and within the residues close to these sites. These findings confirm that the antigenicity of the sites is inherent in their three-dimensional locations.
机译:最近用兔和山羊抗血清确定了精鲸鲸鱼肌红蛋白的整个抗原结构,这使得可以检查抗体识别的抗原结构是否取决于产生抗血清的物种。同样,通过了解抗原结构,可以根据在抗原位点和位点的环境残基中发生的氨基酸取代的影响,更好地理解决定和影响抗原性的分子因素。在目前的工作中,检查了有鳍鲸,虎鲸,马,黑猩猩,绵羊,山羊,牛,针,内脏,兔,狗,海角狐狸,小鼠和鸡的肌球蛋白与抗血清交叉反应的能力。鲸鱼肌红蛋白。通过用放射性碘标记的抗体进行的免疫吸附滴定研究,这些肌球蛋白均能够将抗体与在山羊,兔,鸡,猫,猪和近交小鼠中饲养的精液鲸鱼肌红蛋白结合。发现给定的肌红蛋白的交叉反应程度不取决于产生抗血清的种类。这表明对精子鲸肌红蛋白的抗体反应(即其抗原结构)独立于其中产生抗血清的物种,并且不针对注射的肌红蛋白和免疫宿主的肌红蛋白之间的序列差异区域。实际上,在检查的给定物种的每种抗血清中,该抗血清与该物种的肌红蛋白反应。对于给定的肌红蛋白,这种自身反应的程度与该肌红蛋白与在其他物种中产生的抗血清所显示的交叉反应的一般程度是可比的。交叉反应性和自反应性(对于给定的肌红蛋白而言,两者的程度都相似)可以根据抗原位点内和这些位点附近残基内的氨基酸取代作用而合理地合理化。这些发现证实了位点的抗原性在其三维位置是固有的。

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