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The RNA Transport Element of the Murine musD Retrotransposon Requires Long-range Intramolecular Interactions for Function*

机译:小鼠musD反转录转座子的RNA转运元件需要长距离的分子内相互作用才能发挥功能*

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摘要

Retrovirus replication requires specialized transport mechanisms to export genomic mRNA from the nucleus to the cytoplasm of the infected cell. This regulation is mediated by a combination of viral and/or cellular factors that interact with cis-acting RNA export elements linking the viral RNA to the cellular CRM1 or NXF1 nuclear export pathways. Endogenous type D murine LTR retrotransposons (musD) were reported to contain an RNA export element located upstream of the 3′-LTR. Although functionally equivalent, the musD export element, termed the musD transport element, is distinct from the other retroviral RNA export elements, such as the constitutive transport element of simian/Mason-Pfizer monkey retroviruses and the RNA transport element found in rodent intracisternal A-particle LTR retrotransposons. We demonstrate here that the minimal RNA transport element (musD transport element) of musD comprises multiple secondary structure elements that presumably serve as recognition signals for the cellular export machinery. We identified two classes of tertiary interactions, namely kissing loops and a pseudoknot. This work constitutes the first example of an RNA transport element requiring such structural motifs to mediate nuclear export.
机译:逆转录病毒复制需要专门的转运机制才能将基因组mRNA从细胞核输出到感染细胞的细胞质中。这种调节是由病毒和/或细胞因子的组合介导的,这些因子与将病毒RNA与细胞CRM1或NXF1核输出途径相连的顺式作用RNA输出元件相互作用。据报道,内源性D型鼠LTR反转录转座子(musD)包含位于3'-LTR上游的RNA出口元件。尽管在功能上是等效的,但musD出口元件(称为musD转运元件)与其他逆转录病毒RNA出口元件(例如猿猴/梅森-辉瑞猴逆转录病毒的组成性转运元件)和啮齿动物脑池内A-中的RNA转运元件不同。颗粒LTR逆转座子。在这里,我们证明了musD的最小RNA转运元件(musD转运元件)包括多个二级结构元件,这些二级结构元件可能充当细胞输出设备的识别信号。我们确定了三级相互作用的两类,即亲吻环和假结。这项工作构成了RNA转运元件的第一个例子,该元件需要这种结构基序来介导核输出。

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