首页> 外文OA文献 >Analysis of the transcriptional unit encoding the genes for rubredoxin (rub) and a putative rubredoxin oxidoreductase (rbo) in Desulfovibrio vulgaris Hildenborough.
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Analysis of the transcriptional unit encoding the genes for rubredoxin (rub) and a putative rubredoxin oxidoreductase (rbo) in Desulfovibrio vulgaris Hildenborough.

机译:分析寻常型脱硫弧菌Hildenborough中的氧化还原酶(rub)和推定的氧化还原酶氧化还原酶(rbo)基因的转录单位。

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摘要

The nucleotide sequence of a 2.0-kilobase-pair EcoRI restriction fragment upstream from the gene (rub, 162 base pairs) encoding rubredoxin from Desulfovibrio vulgaris Hildenborough indicates that it is part of a larger transcriptional unit, containing an additional 378-base-pair open reading frame which terminates 16 nucleotides from the translational start of the rub gene and could encode a polypeptide of 14 kilodaltons (kDa). Northern (RNA) blotting of RNA isolated from both D. vulgaris Hildenborough and Escherichia coli TG2 transformed with plasmid pJK29, which contains both genes on a 1.1-kilobase-pair SalI insert, confirms that the genes for this 14-kDa polypeptide and rubredoxin are present on a single transcript of 680 nucleotides. Strong evidence that the 14-kDa polypeptide is also a redox protein is provided by the fact that its NH2 terminus is homologous to desulforedoxin, which has been isolated from D. gigas as a small dimeric redox protein (36 amino acids per monomer), coordinating two iron atoms. Since rubredoxin is a potential redox partner for the 14-kDa protein, it has been tentatively named rubredoxin oxidoreductase, produced by the rbo gene. Southern blotting indicates that the rbo-rub operon is present in several species and strains of sulfate-reducing bacteria.
机译:在来自寻常型脱硫弧菌希尔德伯勒的编码redredoxin的基因(rub,162个碱基对)的上游有一个2.0碱基对的EcoRI限制性片段的核苷酸序列,表明它是一个较大的转录单位的一部分,另外还有378个碱基对开放读框,其从rub基因的翻译起始处终止16个核苷酸,并且可以编码14千道尔顿(kDa)的多肽。 RNA印迹(Northern(RNA)印迹)从质粒pJK29转化的普通D. Vulgaris Hildenborough和大肠杆菌TG2分离的RNA包含在1.1碱基对SalI插入片段中的两个基因,证实该14 kDa多肽和rubredoxin的基因是存在于680个核苷酸的单个转录本上。有力的证据表明14-kDa多肽也是氧化还原蛋白,这是由于其NH2末端与去硫毒素(D. gigas)分离的,它是一种小的二聚体氧化还原蛋白(每个单体36个氨基酸),与两个铁原子。由于rubredoxin是14 kDa蛋白的潜在氧化还原伴侣,因此它被暂时命名为rbo基因产生的rubredoxin氧化还原酶。 Southern印迹表明,rbo-rub操纵子存在于数种硫酸盐还原细菌中。

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  • 作者

    Brumlik, M J; Voordouw, G;

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  • 年度 1989
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  • 原文格式 PDF
  • 正文语种 en
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