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Quorum sensing in Vibrio fischeri: probing autoinducer-LuxR interactions with autoinducer analogs.

机译:费氏弧菌的群体感应:探测自诱导物-LuxR与自诱导物类似物的相互作用。

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摘要

The Vibrio fischeri luminescence genes are activated by the transcription factor LuxR in combination with a diffusible signal compound, N-(3-oxohexanoyl) homoserine lactone, termed the autoinducer. We have synthesized a set of autoinducer analogs. Many analogs with alterations in the acyl side chain showed evidence of binding to LuxR. Some appeared to bind with an affinity similar to that of the autoinducer, but none showed a higher affinity, and many did not bind as tightly as the autoinducer. For the most part, compounds with substitutions in the homoserine lactone ring did not show evidence of binding to LuxR. The exceptions were compounds with a homocysteine thiolactone ring in place of the homoserine lactone ring. Many but not all of the analogs showing evidence of LuxR binding had some ability to activate the luminescence genes. None were as active as the autoinducer. While most showed little ability to induce luminescence, a few analogs with rather conservative substitutions had appreciable activity. Under the conditions we employed, some of the analogs showing little or no ability to induce luminescence were inhibitors of the autoinducer.
机译:费氏弧菌发光基因被转录因子LuxR与可扩散信号化合物N-(3-氧代己酰基)高丝氨酸内酯(称为自体诱导剂)结合激活。我们合成了一组自动诱导物类似物。酰基侧链发生变化的许多类似物均显示出与LuxR结合的证据。一些似乎以与自诱导剂相似的亲和力结合,但是没有一个显示出更高的亲和力,并且许多不像自诱导剂那样紧密结合。在大多数情况下,高丝氨酸内酯环上具有取代基的化合物没有显示出与LuxR结合的证据。具有高半胱氨酸硫代内酯环代替高丝氨酸内酯环的化合物除外。许多但并非全部显示LuxR结合证据的类似物具有激活发光基因的能力。没有一个像自动诱导剂那么活跃。尽管大多数显示出很少的诱导发光的能力,但是一些具有相当保守取代的类似物具有明显的活性。在我们采用的条件下,一些几乎没有或没有诱导发光能力的类似物是自诱导剂的抑制剂。

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