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Photosynthetic and Phylogenetic Primers for Detection of Anoxygenic Phototrophs in Natural Environments

机译:光合和系统发生底漆,用于检测自然环境中的产氧营养养分

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摘要

Primer sets were designed to target specific 16S ribosomal DNA (rDNA) sequences of photosynthetic bacteria, including the green sulfur bacteria, the green nonsulfur bacteria, and the members of the Heliobacteriaceae (a gram-positive phylum). Due to the phylogenetic diversity of purple sulfur and purple nonsulfur phototrophs, the 16S rDNA gene was not an appropriate target for phylogenetic rDNA primers. Thus, a primer set was designed that targets the pufM gene, encoding the M subunit of the photosynthetic reaction center, which is universally distributed among purple phototrophic bacteria. The pufM primer set amplified DNAs not only from purple sulfur and purple nonsulfur phototrophs but also from Chloroflexus species, which also produce a reaction center like that of the purple bacteria. Although the purple bacterial reaction center structurally resembles green plant photosystem II, the pufM primers did not amplify cyanobacterial DNA, further indicating their specificity for purple anoxyphototrophs. This combination of phylogenetic- and photosynthesis-specific primers covers all groups of known anoxygenic phototrophs and as such shows promise as a molecular tool for the rapid assessment of natural samples in ecological studies of these organisms.
机译:设计引物组以靶向光合细菌的特定16S核糖体DNA(rDNA)序列,包括绿色硫细菌,绿色非硫细菌和Heliobacteriaceae(革兰氏阳性菌)的成员。由于紫色硫和紫色非硫光养菌的系统发育多样性,因此16S rDNA基因不是适合系统发育rDNA引物的靶标。因此,设计了针对pufM基因的引物组,该基因编码光合作用中心的M亚基,该基因普遍分布在紫色光养细菌中。 pufM引物组不仅从紫色硫和紫色非硫营养养分体中扩增DNA,而且还从绿屈挠菌种中扩增DNA,后者也产生类似于紫色细菌的反应中心。尽管紫色细菌反应中心在结构上类似于绿色植物光系统II,但pufM引物并未扩增蓝细菌DNA,进一步表明了它们对紫色厌氧营养菌的特异性。系统发育特异性引物和光合作用特异性引物的组合涵盖了所有已知的产氧性光养菌,因此显示出有望作为一种分子工具,用于在这些生物的生态学研究中快速评估天然样品。

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