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Mechanism of DNA Compaction by Yeast Mitochondrial Protein Abf2p

机译:酵母线粒体蛋白Abf2p压缩DNA的机制

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摘要

We used high-resolution atomic force microscopy to image the compaction of linear and circular DNA by the yeast mitochondrial protein Abf2p, which plays a major role in packaging mitochondrial DNA. Atomic force microscopy images show that protein binding induces drastic bends in the DNA backbone for both linear and circular DNA. At a high concentration of Abf2p DNA collapses into a tight nucleoprotein complex. We quantified the compaction of linear DNA by measuring the end-to-end distance of the DNA molecule at increasing concentrations of Abf2p. We also derived a polymer statistical mechanics model that provides a quantitative description of compaction observed in our experiments. This model shows that sharp bends in the DNA backbone are often sufficient to cause DNA compaction. Comparison of our model with the experimental data showed excellent quantitative correlation and allowed us to determine binding characteristics for Abf2p. These studies indicate that Abf2p compacts DNA through a simple mechanism that involves bending of the DNA backbone. We discuss the implications of such a mechanism for mitochondrial DNA maintenance and organization.
机译:我们使用高分辨率原子力显微镜对酵母线粒体蛋白Abf2p压缩线性和环状DNA进行成像,该蛋白在包装线粒体DNA中起主要作用。原子力显微镜图像显示,蛋白质结合会在线性和环状DNA的DNA主链中引起急剧的弯曲。高浓度的Abf2p DNA会塌陷成紧密的核蛋白复合物。我们通过测量Abf2p浓度增加时DNA分子的端对端距离来量化线性DNA的紧密度。我们还导出了聚合物统计力学模型,该模型提供了在实验中观察到的压实度的定量描述。该模型表明,DNA主链的急剧弯曲通常足以引起DNA压缩。我们的模型与实验数据的比较显示出极好的定量相关性,使我们能够确定Abf2p的结合特性。这些研究表明,Abf2p通过涉及DNA骨架弯曲的简单机制来压缩DNA。我们讨论线粒体DNA维护和组织这种机制的含义。

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