首页> 外文OA文献 >Sodium ion/L-lactate co-transport in rabbit small-intestinal brush-border-membrane vesicles.
【2h】

Sodium ion/L-lactate co-transport in rabbit small-intestinal brush-border-membrane vesicles.

机译:钠/ L-乳酸在兔小肠刷状边界膜小泡中共转运。

代理获取
本网站仅为用户提供外文OA文献查询和代理获取服务,本网站没有原文。下单后我们将采用程序或人工为您竭诚获取高质量的原文,但由于OA文献来源多样且变更频繁,仍可能出现获取不到、文献不完整或与标题不符等情况,如果获取不到我们将提供退款服务。请知悉。

摘要

Uptake of L-lactate into rabbit jejunal brush-border-membrane vesicles prepared by a Ca2+-precipitation procedure was studied by a rapid filtration technique with L-[14C]-lactate as tracer. Transport of L-lactate into an intravesicular (osmotically reactive) space could be established. An inwardly directed NaCl gradient (outside 21 mM/inside 0mM) stimulated the uptake of L-lactate at 15 s 2-4-fold compared with that observed with an equal KCl gradient. A transient accumulation of L-lactate inside the vesicles (overshoot) was observed in the presence of an NaCl gradient. Gradients of LiCl, RbCl, CsCl or choline chloride were not able to replace NaCl in the stimulation of L-lactate uptake. L-Lactate uptake was saturable only in the presence of Na+. D-Lactate, DL-thiolactate (2-DL-mercaptopropionate), pyruvate and propionate inhibited the Na+-stimulated L-lactate uptake; D-lactate, thiolactate and pyruvate provoked trans-stimulation of L-lactate uptake. Artificially imposed diffusion potentials (inside negative) did not exert any effect on the Na+-dependent L-lactate uptake. The results are consistent with the existence of an electroneutral Na+/L-lactate co-transport system in the brush border of rabbit small intestine.
机译:采用快速过滤技术,以L- [14C]-乳酸盐为示踪剂,研究了Ca2 +沉淀法制备的兔空肠刷状边界膜囊泡中L-乳酸盐的吸收。可以建立L-乳酸向囊内(渗透反应性)空间的运输。向内定向的NaCl梯度(外部21 mM /内部0mM)与在相同KCl梯度下观察到的刺激相比,在15 s 2-4倍刺激了L-乳酸的摄取。在NaCl梯度存在下,观察到L-乳酸在囊泡中的短暂积累(过冲)。 LiCl,RbCl,CsCl或氯化胆碱的梯度不能代替NaCl刺激L-乳酸的摄取。仅在Na +存在下,L-乳酸的摄取才是饱和的。 D-乳酸,DL-巯基乳酸(2-DL-巯基丙酸酯),丙酮酸和丙酸酯抑制Na +刺激的L-乳酸的摄取。 D-乳酸,巯基乳酸和丙酮酸引起L-乳酸摄取的反式刺激。人工施加的扩散势(负值内)对依赖Na +的L-乳酸摄取没有任何影响。该结果与兔小肠刷状缘中存在电中性Na + / L-乳酸共转运系统一致。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
代理获取

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号