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NS1′ of Flaviviruses in the Japanese Encephalitis Virus Serogroup Is a Product of Ribosomal Frameshifting and Plays a Role in Viral Neuroinvasiveness▿

机译:日本脑炎病毒血清群中黄病毒的NS1'是核糖体移码的产物,并在病毒性神经侵袭中起作用▿

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摘要

Flavivirus NS1 is a nonstructural protein involved in virus replication and regulation of the innate immune response. Interestingly, a larger NS1-related protein, NS1′, is often detected during infection with the members of the Japanese encephalitis virus serogroup of flaviviruses. However, how NS1′ is made and what role it performs in the viral life cycle have not been determined. Here we provide experimental evidence that NS1′ is the product of a −1 ribosomal frameshift event that occurs at a conserved slippery heptanucleotide motif located near the beginning of the NS2A gene and is stimulated by a downstream RNA pseudoknot structure. Using site-directed mutagenesis of these sequence elements in an infectious clone of the Kunjin subtype of West Nile virus, we demonstrate that NS1′ plays a role in viral neuroinvasiveness.
机译:黄病毒NS1是一种非结构蛋白,参与病毒复制和先天免疫应答的调控。有趣的是,在感染黄病毒的日本脑炎病毒血清群成员期间,经常检测到较大的NS1相关蛋白NS1'。然而,尚未确定NS1'的制备方法及其在病毒生命周期中的作用。在这里,我们提供实验证据,证明NS1'是-1核糖体移码事件的产物,该事件发生在位于NS2A基因起点附近的保守的滑七核苷酸基序处,并受到下游RNA假结结构的刺激。在西尼罗河病毒昆金亚型的感染性克隆中,使用这些序列元件的定点诱变,我们证明了NS1'在病毒神经侵袭中起作用。

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