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DNA Microarray-Mediated Transcriptional Profiling of the Escherichia coli Response to Hydrogen Peroxide

机译:DNA芯片介导的大肠杆菌对过氧化氢的转录分析

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摘要

The genome-wide transcription profile of Escherichia coli cells treated with hydrogen peroxide was examined with a DNA microarray composed of 4,169 E. coli open reading frames. By measuring gene expression in isogenic wild-type and oxyR deletion strains, we confirmed that the peroxide response regulator OxyR activates most of the highly hydrogen peroxide-inducible genes. The DNA microarray measurements allowed the identification of several new OxyR-activated genes, including the hemH heme biosynthetic gene; the six-gene suf operon, which may participate in Fe-S cluster assembly or repair; and four genes of unknown function. We also identified several genes, including uxuA, encoding mannonate hydrolase, whose expression might be repressed by OxyR, since their expression was elevated in the ΔoxyR mutant strain. In addition, the induction of some genes was found to be OxyR independent, indicating the existence of other peroxide sensors and regulators in E. coli. For example, the isc operon, which specifies Fe-S cluster formation and repair activities, was induced by hydrogen peroxide in strains lacking either OxyR or the superoxide response regulators SoxRS. These results expand our understanding of the oxidative stress response and raise interesting questions regarding the nature of other regulators that modulate gene expression in response to hydrogen peroxide.
机译:用由4,169个大肠杆菌开放阅读框组成的DNA微阵列检查了用过氧化氢处理的大肠杆菌细胞的全基因组转录谱。通过测量等基因野生型和oxyR缺失菌株中的基因表达,我们证实了过氧化物反应调节剂OxyR激活了大多数高度过氧化氢诱导的基因。 DNA微阵列测量可以鉴​​定出几个新的OxyR激活基因,包括hemH血红素生物合成基因。六基因suf操纵子,可参与Fe-S团簇的组装或修复;和四个功能未知的基因。我们还鉴定了几个编码甘露聚糖水解酶的基因,包括uxuA,其表达可能被OxyR抑制,因为它们的表达在ΔoxyR突变株中升高了。另外,发现某些基因的诱导是与OxyR无关的,这表明大肠杆菌中存在其他过氧化物传感器和调节剂。例如,在缺乏OxyR或超氧化物反应调节剂SoxRS的菌株中,过氧化氢诱导了isc操纵子,该操纵子指定了Fe-S团簇的形成和修复活性。这些结果扩大了我们对氧化应激反应的理解,并引起了其他有趣的问题,这些问题涉及其他调节过氧化氢基因表达的调节剂的性质。

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