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Isolation of two closely related vitellogenin genes, including their flanking regions, from a Xenopus laevis gene library.

机译:从非洲爪蟾基因文库中分离两个密切相关的卵黄蛋白原基因,包括其侧翼区域。

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摘要

A gene library of Xenopus laevis was constructed from embryonic DNA partially digested with restriction endonucleases Hae III and Alu I and joined to the phage lambda Charon 4 cloning vector with EcoRI linkers. Nucleotide sequences from three of the four related vitellogenin genes have been isolated. Two of the genes (called A1 and A2) were isolated in their entirety together with long stretches of flanking sequences. These two closely related vitellogenin genes have lengths of about 21 and 16 kilobases, but both produce a vitellogenin mRNA of 6.3 kilobases.
机译:根据用限制性核酸内切酶Hae III和Alu I部分消化的胚胎DNA构建非洲爪蟾的基因文库,并将其与具有EcoRI接头的噬菌体λCharon 4克隆载体连接。从四个相关的卵黄蛋白原基因中的三个已分离出核苷酸序列。完整地分离了两个基因(称为A1和A2)以及较长的侧翼序列。这两个紧密相关的卵黄蛋白原基因的长度约为21和16千个碱基,但两者均产生6.3千卵黄的卵黄素原mRNA。

著录项

  • 作者

    Wahli, W; Dawid, I B;

  • 作者单位
  • 年度 1980
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  • 原文格式 PDF
  • 正文语种 en
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