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Performance of the Chromogenic Medium CHROMagar Staph Aureus and the Staphychrom Coagulase Test in the Detection and Identification of Staphylococcus aureus in Clinical Specimens

机译:生色培养基CHROMagar金黄色葡萄球菌和Staphychrom凝固酶试验在临床标本金黄色葡萄球菌的检测和鉴定中的性能

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摘要

CHROMagar Staph aureus (CSAM) (CHROMagar Microbiology, Paris, France) is a new chromogenic medium designed to enable detection of colonies of Staphylococcus aureus by their pink color. A total of 775 specimens were cultured in parallel on CHROMagar Staph aureus and conventional media. Among the 267 S. aureus strains recovered on at least one medium, 263 were isolated on CSAM medium (sensitivity, 98.5%), and 245 (sensitivity, 91.8%) were isolated on conventional media. The specificity of presumptive identification of S. aureus on the basis of pink colony color on CSAM medium was 97% (493 of 508). This specificity increased to 100% when coagulase detection with the Staphychrom coagulase test was added and to 98.8% when S. aureus surface components were detected by agglutination in the Pastorex Staph Plus test. Susceptibility testing of 67 S. aureus strains, performed in parallel on pink CSAM colonies and on colonies grown on blood agar, gave similar results. Thus, rapid and accurate recognition and identification of S. aureus isolates were achieved with CSAM as the primary isolation medium, followed by the staphylocoagulase Staphychrom test. Antimicrobial susceptibility testing (disk-diffusion method or ATB STAPH System) can be performed directly on pink CSAM colonies.
机译:CHROMagar Staph aureus(CSAM)(CHROMagar Microbiology,法国巴黎)是一种新型生色培养基,旨在通过其粉红色来检测金黄色葡萄球菌的菌落。总共775个标本在CHROMagar Staph aureus和常规培养基上平行培养。在至少一种培养基上回收的267株金黄色葡萄球菌菌株中,在CSAM培养基上分离出263株(灵敏度为98.5%),在常规培养基上分离出245株(灵敏度为91.8%)。根据CSAM培养基上的粉红色菌落颜色推测金黄色葡萄球菌的特异性为97%(508的493)。当添加用Staphychrom凝固酶检测法进行凝固酶检测时,该特异性增加到100%;当在Pastorex Staph Plus检测中通过凝集法检测到金黄色葡萄球菌表面成分时,该特异性增加到98.8%。在粉红色的CSAM菌落和在血琼脂上生长的菌落并行进行的67株金黄色葡萄球菌菌株的药敏试验得到了相似的结果。因此,以CSAM作为主要分离介质,随后进行了葡萄球菌凝固酶Staphychrom试验,可以快速,准确地识别和鉴定金黄色葡萄球菌。可以在粉红色的CSAM菌落上直接进行抗菌药敏试验(磁盘扩散法或ATB STAPH系统)。

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