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cDNA sequence, protein structure, and chromosomal location of the human gene for poly(ADP-ribose) polymerase.

机译:聚(ADP-核糖)聚合酶的人类基因的cDNA序列,蛋白质结构和染色体位置。

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摘要

Recently we described a full-length cDNA for the human nuclear enzyme poly(ADP-ribose) polymerase. Here, we report the chromosomal localization and partial map of the human gene for this enzyme as well as the complete coding sequence for this protein. The nucleotide sequence reveals a single 3042-base open reading frame encoding a protein with a predicted Mr of 113,135. A comparison of this deduced amino acid sequence with the amino acid sequence of three peptides derived from human poly(ADP-ribose) polymerase revealed a match of 27 amino acid residues. A computer-derived structural analysis of the enzyme and a search for similarities with other proteins confirmed that the polymerase belongs to a subfamily of DNA/NAD-binding proteins and DNA-repair proteins. Possible Zn2+-binding "fingers," a nucleotide-binding fold, and a nuclear transport signal were noted. Additionally, chromosomal mapping has identified polymerase-hybridizing sequences on human chromosomes 1 (the active gene), 13, and 14 (processed pseudogenes). Using the polymerase cDNA as a probe, we also have detected several DNA restriction fragment length polymorphisms in normal humans.
机译:最近,我们描述了人类核酶聚(ADP-核糖)聚合酶的全长cDNA。在这里,我们报告该酶的人类基因的染色体定位和部分图以及该蛋白的完整编码序列。核苷酸序列揭示了一个单一的3042个碱基的开放阅读框,该框编码一个预测的Mr为113135的蛋白质​​。该推导的氨基酸序列与源自人聚(ADP-核糖)聚合酶的三个肽的氨基酸序列的比较显示出27个氨基酸残基的匹配。该酶的计算机衍生结构分析和与其他蛋白质的相似性研究证实,聚合酶属于DNA / NAD结合蛋白和DNA修复蛋白的亚家族。记录了可能的Zn2 +结合“手指”,核苷酸结合倍数和核转运信号。此外,染色体作图已经鉴定了人类染色体1(活性基因),13和14(加工的假基因)上的聚合酶杂交序列。使用聚合酶cDNA作为探针,我们还检测了正常人的几种DNA限制性片段长度多态性。

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