首页> 外文OA文献 >The transcription factor YY1 binds to negative regulatory elements in the human cytomegalovirus major immediate early enhancer/promoter and mediates repression in non-permissive cells.
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The transcription factor YY1 binds to negative regulatory elements in the human cytomegalovirus major immediate early enhancer/promoter and mediates repression in non-permissive cells.

机译:转录因子YY1与人巨细胞病毒主要立即早期增强子/启动子中的负调控元件结合,并介导非许可细胞中的阻遏。

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摘要

We have previously shown that repression of human cytomegalovirus (HCMV) major immediate early (IE) gene expression in non-permissive human teratocarcinoma (T2) cells is associated with a number of nuclear factors which bind to the imperfect dyad symmetry located in the modulator region upstream of the major IE enhancer as well as to the 21 bp repeat elements within the enhancer. Differentiation of T2 cells with retinoic acid (RA) results in a decrease in binding of some of these nuclear factors to these sites and deletion of these specific binding sites from major IE promoter/reporter constructs results in increased IE promoter activity in normally non-permissive cells. In this study, we demonstrate that the transcription factor YY1, which can negatively regulate the adeno-associated virus P5 promoter, directly binds to both the imperfect dyad symmetry and the 21 bp repeat elements in the HCMV major IE promoter/regulatory region and mediates repression of HCMV IE gene expression. This strongly suggests that YY1 plays an important role in regulating HCMV expression in non-permissive cells.
机译:先前我们已经表明,在非允许性人畸胎瘤(T2)细胞中抑制人巨细胞病毒(HCMV)主要立即早期(IE)基因表达与许多核因子相关,这些核因子与位于调节区的不完全二联对称性结合主要IE增强子的上游,以及增强子内21 bp的重复元件。用视黄酸(RA)分化T2细胞会导致其中一些核因子与这些位点的结合减少,并且从主要IE启动子/报告子构建体中删除这些特异性结合位点会导致IE启动子活性增加(通常是不允许的)细胞。在这项研究中,我们证明了可以负调控腺相关病毒P5启动子的转录因子YY1直接与HCMV主要IE启动子/调控区中不完美的对偶对称和21 bp重复元件结合,并介导阻遏HCMV IE基因表达的检测。这有力地表明,YY1在调节非允许细胞中的HCMV表达中起重要作用。

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