首页> 外文OA文献 >Influence of Soil Components on the Biodegradation of Benzene, Toluene, Ethylbenzene, and o-, m-, and p-Xylenes by the Newly Isolated Bacterium Pseudoxanthomonas spadix BD-a59 ▿
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Influence of Soil Components on the Biodegradation of Benzene, Toluene, Ethylbenzene, and o-, m-, and p-Xylenes by the Newly Isolated Bacterium Pseudoxanthomonas spadix BD-a59 ▿

机译:土壤成分对新分离的细菌假单胞菌茎线虫BD-a59对苯,甲苯,乙苯和邻,间和对二甲苯生物降解的影响▿

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摘要

A bacterium designated strain BD-a59, able to degrade all six benzene, toluene, ethylbenzene, and o-, m-, and p-xylene (BTEX) compounds, was isolated by plating gasoline-contaminated sediment from a gasoline station in Geoje, Republic of Korea, without enrichment, on minimal salts basal (MSB) agar containing 0.01% yeast extract, with BTEX as the sole carbon and energy source. Taxonomic analyses showed that the isolate belonged to Pseudoxanthomonas spadix, and until now, the genus Pseudoxanthomonas has not included any known BTEX degraders. The BTEX biodegradation rate was very low in MSB broth, but adding a small amount of yeast extract greatly enhanced the biodegradation. Interestingly, degradation occurred very quickly in slurry systems amended with sterile soil solids but not with aqueous soil extract. Moreover, if soil was combusted first to remove organic matter, the enhancement effect on BTEX biodegradation was lost, indicating that some components of insoluble organic compounds are nutritionally beneficial for BTEX degradation. Reverse transcriptase PCR-based analysis of field-fixed mRNA revealed expression of the tmoA gene, whose sequence was closely related to that carried by strain BD-a59. This study suggests that strain BD-a59 has the potential to assist in BTEX biodegradation at contaminated sites.
机译:通过在巨济市的一家加油站镀上被汽油污染的沉积物,分离出了能够降解所有六种苯,甲苯,乙苯和邻,间和对二甲苯(BTEX)化合物的细菌BD-a59菌株。大韩民国,不富集,使用含有0.01%酵母提取物的基本盐基础(MSB)琼脂,其中BTEX是唯一的碳和能源。分类学分析表明,该分离物属于假单胞菌茎线虫,到目前为止,假单胞菌属还没有包括任何已知的BTEX降解物。 MSB肉汤中BTEX的生物降解率非常低,但是添加少量酵母提取物可以大大提高生物降解性。有趣的是,在用无菌土壤固体改良的浆液系统中降解很快发生,而在水性土壤提取物上却没有。此外,如果先燃烧土壤以去除有机物,则对BTEX生物降解的增强作用就消失了,这表明不溶性有机化合物的某些成分在营养上对BTEX降解有益。基于逆转录酶PCR的现场固定mRNA分析显示tmoA基因的表达,其序列与菌株BD-a59的序列密切相关。这项研究表明,菌株BD-a59有潜力协助受污染部位的BTEX生物降解。

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