首页> 外文OA文献 >Characterization of a cDNA encoding a cysteine-rich cell surface protein located in the flagellar pocket of the protozoan Trypanosoma brucei.
【2h】

Characterization of a cDNA encoding a cysteine-rich cell surface protein located in the flagellar pocket of the protozoan Trypanosoma brucei.

机译:位于原生动物Trypanosoma brucei鞭毛袋中的富含半胱氨酸的细胞表面蛋白编码cDNA的表征。

代理获取
本网站仅为用户提供外文OA文献查询和代理获取服务,本网站没有原文。下单后我们将采用程序或人工为您竭诚获取高质量的原文,但由于OA文献来源多样且变更频繁,仍可能出现获取不到、文献不完整或与标题不符等情况,如果获取不到我们将提供退款服务。请知悉。

摘要

We have characterized a cDNA encoding a cysteine-rich, acidic integral membrane protein (CRAM) of the parasitic protozoa Trypanosoma brucei and Trypanosoma equiperdum. Unlike other membrane proteins of T. brucei, which are distributed throughout the cell surface, CRAM is concentrated in the flagellar pocket, an invagination of the cell surface of the trypanosome where endocytosis has been documented. Accordingly, CRAM also locates to vesicles located underneath the pocket, providing evidence of its internalization. CRAM has a predicted molecular mass of 130 kilodaltons and has a signal peptide, a transmembrane domain, and a 41-amino-acid cytoplasmic extension. A characteristic feature of CRAM is a large extracellular domain with a roughly 66-fold acidic, cysteine-rich 12-amino-acid repeat. CRAM is conserved among different protozoan species, including Trypanosoma cruzi, and CRAM has structural similarities with eucaryotic cell surface receptors. The most striking homology of CRAM is to the human low-density-lipoprotein receptor. We propose that CRAM functions as a cell surface receptor of different trypanosome species.
机译:我们已表征了编码寄生虫原生动物锥虫锥虫和马氏锥虫的富含半胱氨酸,酸性整合膜蛋白(CRAM)的cDNA。与布氏锥虫的其他膜蛋白分布在整个细胞表面不同,CRAM集中在鞭毛袋中,这是锥虫体细胞表面的内陷,已被证明具有内吞作用。因此,CRAM也位于囊袋下方的囊泡中,提供了其内在化的证据。 CRAM的预测分子量为130道尔顿,并具有信号肽,跨膜结构域和41个氨基酸的胞质延伸。 CRAM的一个特征是大的细胞外结构域,具有大约66倍的酸性,富含半胱氨酸的12个氨基酸重复序列。 CRAM在包括原生锥虫(Trypanosoma cruzi)在内的不同原生动物物种中都是保守的,并且CRAM与真核细胞表面受体具有结构相似性。 CRAM最惊人的同源性是与人类低密度脂蛋白受体的同源性。我们建议,CRAM充当不同锥虫物种的细胞表面受体。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
代理获取

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号