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Genes involved in lipopolysaccharide production and symbiosis are clustered on the chromosome of Rhizobium leguminosarum biovar viciae VF39.

机译:涉及脂多糖产生和共生的基因聚集在豆科根瘤菌生物变种蚕豆VF39的染色体上。

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摘要

Four mutants of Rhizobium leguminosarum biovar viciae VF39 altered in lipopolysaccharide (LPS) synthesis were isolated upon random Tn5 mutagenesis. These mutants produced matt colonies on TY medium and showed autoagglutination and loss of motility. On sodium dodecyl sulfate-polyacrylamide gels, they lacked a slow-migrating carbohydrate band, corresponding to the complete LPS (LPSI). All four mutants formed small white nodules on Vicia hirsuta. These nodules were infected but showed no nitrogen-fixing activity and senesced prematurely. Three of the mutants were complemented by a wild-type cosmid to synthesis of normal LPS and induction of nitrogen-fixing nodules. By hybridization and in vivo cloning experiments, the mutations were mapped within different EcoRI fragments which could be localized on the VF39 chromosome. Cross-complementation analyses revealed that the three mutants were affected in different transcriptional units. The results indicate that a cluster of genes necessary for LPSI production and symbiotic efficiency is located within a defined region of 20 kilobases on the R. leguminosarum bv. viciae chromosome.
机译:随机Tn5诱变分离出四个在脂多糖(LPS)合成中改变的豆科根瘤菌生物变种VF39突变体。这些突变体在TY培养基上产生亚光菌落,并表现出自凝集作用和丧失活力。在十二烷基硫酸钠-聚丙烯酰胺凝胶上,它们缺少缓慢迁移的糖带,与完整的LPS(LPSI)相对应。所有四个突变体在野豌豆上均形成小的白色结节。这些结节已被感染,但没有固氮活性,过早衰老。其中三个突变体由野生型粘粒进行补充,以合成正常的LPS和诱导固氮结核。通过杂交和体内克隆实验,将突变定位在可以定位在VF39染色体上的不同EcoRI片段内。交叉互补分析表明,这三个突变体在不同的转录单位中受到影响。结果表明,对于LPSI产生和共生效率而言,必需的基因簇位于豆科植物根瘤菌(R. leguminosarum bv)的20公里碱基的定义区域内。蚕豆染色体。

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  • 作者

    Priefer, U B;

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  • 年度 1989
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  • 原文格式 PDF
  • 正文语种 en
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