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Regulation of tabtoxin production by the lemA gene in Pseudomonas syringae.

机译:lemA基因在丁香假单胞菌中调节毒素的产生。

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摘要

Pseudomonas syringae pv. coronafaciens, a pathogen of oats, was mutagenized with Tn5 to generate mutants defective in tabtoxin production. From a screen of 3,400 kanamycin-resistant transconjugants, seven independent mutants that do not produce tabtoxin (Tox-) were isolated. Although the Tn5 insertions within these seven mutants were linked, they were not located in the previously described tabtoxin biosynthetic region of P. syringae. Instead, all of the insertions were within the P. syringae pv. coronafaciens lemA gene. The lemA gene is required by strains of P. syringae pv. syringae for pathogenicity on bean plants (Phaseolus vulgaris). In contrast to the phenotype of a P. syringae pv. syringae lemA mutant, the Tox- mutants of P. syringae pv. coronafaciens were still able to produce necrotic lesions on oat plants (Avena sativa), although without the chlorosis associated with tabtoxin production. Northern (RNA) hybridization experiments indicated that a functional lemA gene was required for the detection of a transcript produced from the tblA locus located in the tabtoxin biosynthetic region. Marker exchange mutagenesis of the tblA locus resulted in loss of tabtoxin production. Therefore, both the tblA and lemA genes are required for tabtoxin biosynthesis, and the regulation of tabtoxin production by lemA probably occurs at the transcriptional level.
机译:丁香假单胞菌PV。用Tn5诱变了燕麦的病原体-冠状冠腐病(Coronafaciens),从而产生了毒素产生缺陷的突变体。从筛选的3,400卡那霉素抗性转导结合体中,分离出七个不产生毒素(Tox-)的独立突变体。尽管这七个突变体中的Tn5插入被链接,但它们不位于丁香假单胞菌的先前描述的毒素生物合成区域中。相反,所有插入都在丁香假单胞菌pv内。 Coronafaciens lemA基因。 lemA基因是丁香假单胞菌pv株所需的。丁香花对豆类植物的致病性(菜豆)。与丁香假单胞菌pv的表型相反。 syringae lemA突变体,丁香假单胞菌pv的Tox突变体。尽管没有与毒素产生相关的萎黄病,但冠状腐殖质仍能在燕麦植物(苜蓿)上产生坏死性病变。 Northern(RNA)杂交实验表明,功能性lemA基因是检测位于毒素生物合成区域的tblA基因座产生的转录本所必需的。 tblA基因座的标记交换诱变导致产生的毒素产生损失。因此,tblA和lemA基因都是禁忌毒素生物合成所必需的,而lemA对禁忌毒素产生的调节可能发生在转录水平。

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