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Induction of a Highly Specific Antiviral State in Transgenic Plants: Implications for Regulation of Gene Expression and Virus Resistance.

机译:转基因植物中高特异性抗病毒状态的诱导:对基因表达和病毒抗性调控的含义。

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摘要

Transgenic tobacco plants expressing either a full-length form of the tobacco etch virus (TEV) coat protein or a form truncated at the N terminus of the TEV coat protein were initially susceptible to TEV infection, and typical systemic symptoms developed. However, 3 to 5 weeks after a TEV infection was established, transgenic plants "recovered" from the TEV infection, and new stem and leaf tissue emerged symptom and virus free. A TEV-resistant state was induced in the recovered tissue. The resistance was virus specific. Recovered plant tissue could not be infected with TEV, but was susceptible to the closely related virus, potato virus Y. The resistance phenotype was functional at the single-cell level because protoplasts from recovered transgenic tissue did not support TEV replication. Surprisingly, steady state transgene mRNA levels in recovered tissue were 12-to 22-fold less than transgene mRNA levels in uninoculated transgenic tissue of the same developmental stage. However, nuclear run-off studies suggested that transgene transcription rates in recovered and uninoculated plants were similar. We propose that the resistant state and reduced steady state levels of transgene transcript accumulation are mediated at the cellular level by a cytoplasmic activity that targets specific RNA sequences for inactivation.
机译:表达全长形式的烟草蚀刻病毒(TEV)外壳蛋白或在TEV外壳蛋白N末端被截短的形式的转基因烟草植物最初易受TEV感染,并出现了典型的全身症状。然而,TEV感染建立后3至5周,转基因植物从TEV感染中“恢复”,新的茎和叶组织出现症状且无病毒。在恢复的组织中诱导出TEV抗性状态。抗性是病毒特异性的。恢复的植物组织不能被TEV感染,但容易受到密切相关的病毒马铃薯病毒Y的影响。耐药表型在单细胞水平上具有功能,因为从恢复的转基因组织中原生质体不支持TEV复制。出人意料的是,恢复组织中的稳态转基因mRNA水平比相同发育阶段的未接种转基因组织中的转基因mRNA水平低12至22倍。但是,核径流研究表明,恢复和未接种植物中的转基因转录率相似。我们建议,转基因转录物积累的抗性状态和降低的稳态水平在细胞水平上由靶向特定RNA序列进行灭活的细胞质活性介导。

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