Histopathological Comparison of Wound Healing Effect of Various Drugs to Gastric Ulcer of Rat : Verification of Effectiveness of Endoscopic Hemostasis of Gastric Ulcer between the Fibrin Glue and bFGF

摘要

A number of patients who are brought to our emergency center suffer from complication of coagulation-fibrinolysis disorder and those patients who also suffer from gastrointestinal hemorrhage present a problem in endoscopic hemorrhagic control. Thus an experiment was conducted by using rat to find the hemostatic and tissue healing effects of fibrin glue and basic fibrinocyte growth factor (bFGF). Under Nembutal anesthesia, the rats were subjected to laparotomy to create mechanically hemorrhagic ulcers in the pyloric antrum. Into these lesions a physiological saline (sham), Bosmin in a hypertonic saline (HSE), ethanol (ET) or a fibrin glue (FG) was injected locally and their wound were closed. On the 1st, 3rd, 5th and 7th day following surgery, the abdomen was re-opened and pylorectomy was conducted for comparative evaluation of the local hemostatic and ulcer healing effects. FG was most effective in epithelial regeneration, remaining in the tissue until 7th day and suggesting its sustained hemostatic effect. Other groups showed some complications, such as mucosal and submucosal hemorrhage, inflammatory cell infiltration, poor epithelial development and others. The FG local injection therapy results in satisfactory epithelial regeneration: in addition to its hemostatic effect, the wound healing effect may be expected from the combination with bFGF.